I think it is pretty likely that Tremaine is Rapunzel. The question now is just which one of the people left is fake Hook’s daughter.
I think it’s odd they introduced Anastasia right after they announced WishRealm!Hook has a missing daughter and in the same episode they dropped some pretty big Tangled hints. My money is on Anastasia.
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"He was a lot of things to me" "The only conclusion was love"